【摘要】 目的研究肿瘤坏死因子相关凋亡诱导配体受体(TRAILR)在间变性星形细胞瘤中的表达,并探讨其临床意义。方法联合采用免疫组织化学和原位杂交方法检测间变性星形细胞瘤及正常脑组织中TRAILR的表达。结果20例间变性星形细胞瘤均大量表达死亡受体(DR)DR4和DR5,9例(45.0%)表达诱骗受体(DcR)DcR1,5例(25.0%)表达DcR2,而18例正常脑组织普遍表达DcR,7例(38.9%)表达DR4,6例(33.3%)表达DR5。间变性星形细胞瘤组织中DR的高表达以及DcR的低表达不同于正常脑组织中DR的低表达及DcR的高表达,两者比较差异显著(P<0.01)。原位杂交显示,20例间变性星形细胞瘤组织分别有19例(95.0%)DcR1和17例(85.0%)DcR2在mRNA水平呈阳性表达,DcR在转录水平的表达明显高于翻译水平(P<0.01)。结论间变性星形细胞瘤中普遍存在DR的高表达和DcR的低表达,DcR在间变性星形细胞瘤中限制性表达的调控位于转录后水平。更多还原

【Abstract】 Objective To investigate the expression and significance of TNF-related apoptosis-inducing ligand receptor(TRAILR) in human anaplastic astrocytoma.Methods The expression of TRAILR was determined by immunohistochemistry and in situ hybridization in 20 samples of anaplastic astrocytoma and 18 samples of normal brain tissue.Results With low decoy receptor(DcR) expression in partial anaplastic astrocytoma samples and low death receptor(DR) expression in partial normal brain cells,DR was expressed highly in all anaplastic astrocytoma samples while DcR in most normal brain tissue.High DR expression and low DcR expression in anaplastic astrocytoma tissue differed from low DR expression and high DcR expression in normal brain tissue((P<)0.01).We also found there was significant difference between the DcR expression in mRNA level and that in protein level(P<0.01)in anaplastic astrocytoma tissue.Conclusions High DR expression and low DcR expression are prevalent in anaplastic astrocytoma tissue.The control of DcR expression lays in protein level.This may contribute to the apoptosis-inducing therapy of anaplastic astrocytoma.更多还原