Total flavonoids in Ginkgo leaves and its extractive solution were determined by Spectrophotometry . We studied the conditions of determination and the factors of interference.Regression equation was A=11.560 2C + 0.008 5,correlation coefficient r=0.9999,linear range of obeying Bill law :8～80 μg/mL.Content of total flavonoids in Ginkgo leaves was 1.47%.The leaves immerged simply in the 70% alcohol,their best extractive ratio achieved 89.12%.Concentration of extractive solution was 600 μg/mL.
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