【摘要】 研究了用微柱高效液相色谱法测定苦荞中芦丁和槲皮素的方法。苦荞中的芦丁和槲皮素用90%甲醇加热回流提取,以Waters XterraTMRP18(1.0 mm×50 mm,2.5μm)色谱柱为固定相,1%的乙酸和甲醇为流动相,在该色谱条件下,苦荞中芦丁和槲皮素在2.0 min内可达到基线分离,用紫外二极管矩阵检测器检测,方法用于4种苦荞试样中芦丁和槲皮素的测定,分析结果的RSD在0.51%~1.20%之间,回收率在98%~102%间。更多还原

【Abstract】 A high performance liquid chromatographic method with microcolumn for the determination of rutin and quercetin in buckwheat（fagopyrum tataricum） was studied.Rutin and quercetin were extracted from the sample by refluxing with 90% methanol for 30 min.Rutin and quercetin in the extract were separated on Waters Xterra^TM RP₁₈（1.0 mm×50 mm,2.5 μm） chromatographic column with methanol and 1% acetic acid（60∶40） as the mobile phase passing through the column at a flow-rate of 0.2 mL·min^-1,and detected with the photodiode array detector at 360 nm.In the analysis of 4 buckwheat samples by this method,RSD′s obtained were in the range of 0.51%¹.20%,and recoveries in the range of 98%¹02%.更多还原