【摘要】 利用毛细管电泳技术,建立了一种分离、测定磺化杯芳烃的方法。研究了缓冲液浓度和pH、分离电压和进样时间等因素对分离性能的影响。采用磷酸缓冲液(pH 8.7)作分离介质,分离电压15 kV时,6 min内可实现对磺化杯[4]芳烃、磺化杯[6]芳烃和磺化杯[8]芳烃的完全分离。该法充分利用了样品堆积效应,无需任何添加剂,与文献相比简单快速。磺化杯芳烃的浓度(0.001 0~1.0 mmol.L-1)与峰高呈良好的线性关系,其相关系数均优于0.999 3,检出限分别为0.43,0.25和0.32μmol.L-1。更多还原

【Abstract】 A new method based on capillary electrophoresis was developed for the separation and determination of sulfonated calixarenes.Effects of several important factors such as the pH and concentration of buffer,separation voltage and sample injection time were investigated to optimize the conditions.Three sulfonated calixarenes were well separated within 6 min in a 37 cm length capillary at a separation voltage of（15 kV） in a（10 mmol）·L^-1 phosphate buffer（pH 8.7）.Making full use of sample stacking effect,additives were not necessary in this method thus making it is much simpler and faster than that reported in literature.The relationship of peak heights and concentrations was linear over about three orders of magnitude with correlation coefficients over 0.999 3 and detection limits（S/N=3） of 0.43,0.25 and 0.32 μmol·L^-1 for 3 sulfonated calixarenes i.e.sulfonated calixarene[4],sulfonated calixarene[6] and sulfonated calixarene[8],respectively.更多还原