【摘要】 用反相HPLC法测定了银杏叶中黄酮的含量,黄酮甙由70%乙醇-水提取,并用盐酸溶液水解。采用ShimadzuODSC18柱(150mm×6.0mm,5μm),流动相为V甲醇∶V水(0.3%磷酸)=55∶45体系,流速为1mL.min-1,紫外检测器(370nm)。以槲皮素为标准品,在0.0100~0.2000g.L-1范围内,相关系数为0.9995,RSD为0.75%。更多还原

【Abstract】 A reversed phase HPLC method for the determination of flavones in gingko leaves was described in this paper. The flavone glycosides were extracted with 70% ethanol aqueous solution, and hydrolyzed with hydrochloric acid. A shimadzu ODS C₁₈ （150 mm×6.0 mm×5 μm） column was used as the chromatographic column and a mixture of methanol and water （55∶45 by volume） containing 0.3% phosphoric acid used as mobile phase at flow rate of 1 mL·min^-1 for gradient elution. UV detector was set at 370 nm. Quercetin standards were used for calibration. Linear selationship was obtained in range of 0.010 0-0.200 0 g·L^-1 with correlation coefficient （r） of 0.999 5, and with RSD of 0.75%.更多还原