【摘要】 研究了槲皮素 钼(Ⅵ) CTMAB荧光体系的形成条件,并建立了测定槲皮素的荧光光度法。在pH 2.5~3.5的酸度条件下,形成槲皮素∶钼(Ⅵ)∶CTMAB=2∶2∶1 的黄色荧光络合物,激发波长为404.9 nm,发射波长为 472.2 nm,槲皮素浓度在 2.0×10-6 ~1.0×10-5 mol·L-1范围内与荧光强度呈线性关系,检出限为3.4×10-8mol·L-1,所拟方法用于测定中药槐米中的槲皮素,结果满意。更多还原

【Abstract】 The fluorescence reaction of quercetin with Mo（Ⅵ） in the presence of CTMAB was studied and the reaction conditions were optimized. Based on these experimental results, a fluorescence method for the determination of quercetin was established. A yellow fluorescent complex having a mole ratio of （Quercetin∶Mo（Ⅵ）∶CTMAB） 2∶2∶1 is formed in a slightly acidic medium of pH 2.5³.5. Linear relationship between the fluorescence intensity and the concentration of quercetin was obtained in the range of 2.0×10^-61.0×10^-5mol·L^-1 of quercetin and its detection limit was found to be 3.4×10^-8mol·L^-1. Excitation wavelength of 404.9 nm and emission wavelength of 472.2 nm were used in the fluorescence measurement. The method has been applied to the analysis of some Chinese medicines （e.g. Chinese scholartree） with satisfactory results.更多还原