【摘要】 [目的]探讨核转录因子-kappaB(NF-κB)在PM2.5染毒小鼠急性肺损伤过程中的作用。[方法]采集上海市交通区PM2.5,设低(1.6mg/kg)、中(8.0mg/kg)、高(40mg/kg)浓度组和对照组,通过气管滴注染毒C57BL/6小鼠24h后,用RT-PCR方法对肺组织中肿瘤坏死因子α(TNF-α)、白细胞介素1(IL-1)、白细胞介素6(IL-6)、一氧化氮合酶(NOS)和NF-κB的基因表达水平进行检测,观察PM2.5染毒后各指标基因表达的改变情况。[结果]小鼠肺组织中TNF-α、IL-1、IL-6和NOS基因表达水平各染毒组均显著高于对照组(P<0.01),并随着染毒浓度的增加而增加,NF-κB的基因表达水平与上述指标基因水平显著相关,相关系数均高于0.9(P<0.01)。[结论]PM2.5能够引起小鼠肺组织中细胞因子和氧化酶基因水平表达增加,从而造成肺组织的免疫和氧化损伤,NF-κB在PM2.5引起急性肺损伤过程中可能起到重要的调节作用。更多还原

【Abstract】 [ Objective] To explore the role of NF-κB in the course of acute lung injury induced by PM2.5 in mice. [Methods] C57BL/6 mice which are sensitive to PM were randomly divided into four groups. The exposed groups( low,middle and high) were instillated with suspension of PM2.5,and the control group was instillated saline via trachea. 24 hours after instillation mRNA expressions of TNF-α,IL-1,IL-6,NOS and NF-κB in lung were examined with RT-PCR. [ Results] mRNA expressions of TNF-α,IL-1,IL-6,NOS in the instillated groups were higher than those in the control group(P < 0.01),and increased with the dose. Gene expression of NF-κB was related to these genes expressions and the correlation coefficients were more than 0.9(P < 0.01). [ Conclusion] PM2.5 exposure could increase gene expression of cytokines and oxidase of lung,which responsible for immune injury and oxidative injury. NF-κB may have a key role in the course of lung injury induced by PM2.5.更多还原