【摘要】 [目的 ]探讨二英 (TCDD)对人体肝癌细胞株 (HepG2 )和肺腺癌细胞株 (SPC A1)中P5 3基因和P3 8MAPK基因表达的影响 ,并研究剂量与效应的关系。 [方法 ]用常规的细胞培养方法 ,利用RT PCR方法对 2种基因进行表达 ,并以 β actin作为内对照。对基因表达的强度进行比较分析。[结果 ]P3 8MAPK基因在 2种细胞中都有表达 ,在HepG2中 ,P3 8MAPK基因表达随浓度 1、5、10、2 0nmol/L增加先抑后扬 (表达值分别为 0 .90 0、0 .82 0、1.2 60、0 .898,P <0 0 5 ) ,而在SPC A1中差异无显著性 (表达值分别为 0 .898、 0 .919、 0 .80 8、 0 .846,P >0 0 5 )。P5 3基因在人体肝癌细胞株(HepG2 )中有表达 ,并随浓度增加而上调 (表达值分别为 0 .764、0 .890、1.0 99、1.2 18、1.40 5 ,P <0 .0 5 )而在肺腺癌细胞株(SPC A1)中未见表达。 [结论 ]二英对人体肝癌细胞株 (HepG2 )中P5 3基因和P3 8MAPK基因有诱导作用 ,在肺腺癌细胞株 (SPC A1)中仅见对P3 8MAPK基因有诱导作用 ,P5 3蛋白未见阳性表达更多还原

【Abstract】 Objective To study the effects of P53 gene and P38 MAPK gene in HepG2 and SPC-A1 Cells after treatmet with 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD) and their dose-response. The RT-PCR was used to amplify the goal genes in this study and the β-actin of cell lines was used as internal control. The results showed that P38 MAPK gene expressed constitutively in two cell lines,P38 MAPK gene expressed when treated with 1,5,10,20 nmol/L in HepG2 cells in (0.900,0.82,1.26,0.898,P<0.05) when treated,there was no significant difference in SPC-A1 cells (values were 0.898,0.919,0.808,0.846,P>0.05),P53 gene expressed in HepG2 cells increased with concentration (values were 0.764,0.89,1.099,1.218,1.405,P<0.05),and not expressed in SPC-A1 cells. [Conclusion] TCDD can induce P53 gene and P38 MAPK gene in HepG2 cells,and it can induce P38 MAPK gene expression,but not P53 gene,in SPC-A1 cells.更多还原