【摘要】 目的建立一种同时测定磺胺嘧啶麻黄碱滴鼻液中磺胺嘧啶和盐酸麻黄碱含量的方法。方法采用Diamonsil C18色谱柱(150 mm×4.6 mm,5μm),流动相:缓冲液(0.01 mol.L-1磷酸二氢钾,0.5%三乙胺,磷酸调pH至2.8)-甲醇(80∶20,V/V),流速1mL.min-1,柱温25℃,检测波长256 nm。结果在该色谱条件下,盐酸麻黄碱和磺胺嘧啶的线性回归方程分别为y=796.30x+8.01(r=0.999 1,n=6)和y=69.04x-21.10(r=0.999 9,n=6)。加样回收率97%~103%(n=3),RSD<3%(n=3)。样品在8 h内稳定。测得样品中盐酸麻黄碱含量在97%~103%之间,磺胺嘧啶含量在90%~110%之间。结论该方法简便、快速、准确、重现性好。更多还原

【Abstract】 AIM To develop a HPLC method to determine the content of sulfadiazine and ephedrine hydrochloride. METHODS The HPLC separation was carried out on a Diamonsil C₁₈（150 mm×4.6 mm,5 μm） column, using a UV detector and the wavelength was 256 nm.The mobile phase was 0.01mol·L^-1 KH₂PO₄（0.5% triethlamine,adjusted to pH 2.8 with phosphoric acid）-CH₃OH（80∶20,V/V）.The temperature of the column was 25℃. RESULTS Two good linearity was demonstrated by linear equation（r>0.999 1,n=6）.The samples were qualified. CONCLUSION The method is proved to be accurate, sensitive and reliable.更多还原