【摘要】 目的:了解非小细胞肺癌(NSCLC)组织中基质金属蛋白酶-9(MMP-9)和表皮生长因子受体(EGFR)蛋白的表达及其与肺癌组织类型、细胞分化和淋巴结转移的关系。方法:应用S-P免疫组化的方法检测38例非小细胞肺癌组织和10例正常肺组织中MMP-9和EGFR蛋白的表达。结果:38例NSCLC中MMP-9蛋白的阳性表达率为78·9%(30/38),而中度至强阳性的表达率为60·5%(23/38),与正常对照组比较有显著性差别(P<0·05),其中鳞癌、腺癌MMP-9蛋白的阳性表达率分别为60·0%(12/20)和88·9%(16/18)(P<0·05);有纵隔淋巴结转移组肿瘤组织MMP-9阳性表达率93·3%(28/30),显著高于无纵隔淋巴结转移组50·0%(4/8)(P<0·05);中低分化肿瘤组织MMP-9阳性表达率为80·8%(21/26),明显高于高分化肿瘤组织58·3%(7/12)(P<0·05)。EGFR蛋白在38例NSCLC的表达率为57·9%(22/38),在正常肺组织表达为阴性,二者比较有显著性差异(P<0·05),其中鳞癌、腺癌EGFR蛋白的阳性表达率分别为45·0%(9/20)和72·2%(13/18),二者比较无显著性差异(P>0·05)。有纵隔淋巴结转移组EGFR阳性表达率56·7%(17/30),显著高于无淋巴结转移组37·5%(3/8)(P<0·05);中低分化肿瘤组织MMP-9阳性表达率为69·2%(18/26),明显高于高分化肿瘤组织33·3%(4/12)(P<0·05)。MMP-9和EGFR蛋白在Ⅲ期NSCLC中共同表达率占44·7%(17/38),显著高于Ⅰ期和Ⅱ期患者的13·1%(5/38)。结论:NSCLC中MMP-9蛋白的表达与肺癌的病理分型、细胞分化及淋巴结转移有关;EGFR蛋白的表达和肺癌的TNM分期和淋巴结的转移有关;提示EGFR的信号转导与MMP-9在肿瘤浸润和转移过程中发挥重要作用,MMP-9和EGFR的共同高表达是肺癌不良预后的指标。更多还原

【Abstract】 Objective:To investigate the expression and biological significance of MMP-9 and EGFR in 38 NSCLC patients and 10 cases normal lung tissues in order to seek their relationship between them and the prognostic significance.Methods:Tissues were obtained from 38 cases in NSCLC and 10 cases normal lung tissue. The expression of MMP-9 and EGFR were examined by immunohistochemical technique (streptavidin-peroxidase conjugate method, S-P) and the results were correlated with clinical pathological tumor parameters.Results:The positive expression of MMP-9 and EGFR in 38 cases NSCLC were 78.9%(30/38) and 57.9%(22/38) respectively which was significantly higher than that in normal lung tissues (P<0.05). The positive expression of MMP-9 protein was related with histological classification cell differentiation and also related to lymph node metastasis (P<0.05), The positive expression of EGFR protein was not related to histological classification, but it was related to lymph node metastasis and cell differentiation (P<0.05). The rate of MMP-9 protein and EGFR protein co-expression in stage Ⅲ NSCLC was 44.7%(17/38), and the expression of MMP-9 and EGFR was significantly higher than that in stage Ⅰand stage Ⅱ(13.1%,5/38).Conclusion:MMP-9 and EGFR may play important roles in NSCLC invasion and metastasis. Over expression of MMP-9 and EGFR protein in NSCLC could act as a reference to evaluate metastasis and unfavourable prognosis of NSCLC.更多还原