【摘要】 目的检测再生障碍性贫血(再障)患者单个CD8+T细胞内干扰素-γ(IFN-γ)的绝对含量。方法用毛细管电泳免疫分析激光诱导荧光法对再障患者外周血单个CD8+T细胞内IFN-γ的水平进行定量检测。结果2例重型再障(SAA)患者免疫治疗前外周血单个CD8+T细胞内IFN-γ的水平分别为(151.53±28.92)z mol和(223.72±45.23)z mol,免疫治疗后相应的降为(45.57±13.90)z mol和(36.44±9.66)z mol,正常对照为(47.47±17.97)z mol。经统计分析,SAA患者治疗前、后及与正常对照比较差异均有统计学意义。结论毛细管电泳免疫分析激光诱导荧光法可以检测到外周血单个CD8+T细胞内IFN-γ的z mol水平,有望成为一种新的检测方法应用于临床。更多还原

【Abstract】 Objective:to detect the absolute IFN-γ content in single CD8+T cell of patients with SAA.Method:We used CEIA-LIF to detect the IFN-γ level in single CD8+T cell from peripheral blood in patients with SAA.Result:The IFN-γ levels in single CD8+T cell of 2 patients with SAA before immunosuppression treatment were ( 151.53 ± 28.92 ) z mol and ( 223.72 ± 45.23 ) z mol, respectively, then reduced to ( 45.57 ± 13.90 ) z mol and ( 36.44 ± 9.66 ) z mol after treatment. As a control, the normal was 27.47 ± 17.97 z mol. There existed significant difference between the above data.Conclusion:CEIA-LIF can detect the IFN-γ level in single CD8+T cell from peripheral blood in minimum level of zmol, and be expected to be a new method used in clinic.更多还原