【摘要】 目的探讨基质金属蛋白酶9(MMP9)及其组织抑制物(TIMP1)在人结核性淋巴结中的表达及其临床意义。方法免疫组化SP法检测MMP9和TIMP1蛋白在20例活动性淋巴结结核、20例陈旧性结核性淋巴结病灶和20例慢性非特异性淋巴结炎中的表达,采用图象分析技术对免疫组化结果进行定量分析。结果MMP9和TIMP1在三组淋巴结病变均有表达,其中MMP9在活动性淋巴结结核的肉芽肿、坏死周围炎性区呈强表达,在非特异性淋巴结炎和陈旧性结核病灶呈弱表达。MMP9,TIMP1及MMP9/TIMP1在活动性结核组表达显著高于非特异性淋巴结炎和陈旧性结核病灶(均为P<0.01),在单纯活动性淋巴结核组和合并活动性肺结核组表达无显著性差异(均为P>0.05)。结论MMP9、TIMP1高表达可能在结核性淋巴结炎发病机制中发挥重要作用,MMP9/TIMP1失衡可能预示疾病进展和结核播散。更多还原

【Abstract】 Objective To investigate the expression and significance of matrix metalloproteinase-9(MMP-9) and its tissue inhibitor(TIMP-1) in tuberculous lymphadenitis.Methods Immunohistochemical method of streptavidin-peroxidase(SP) was used to detect the expression of MMP-9 and TIMP-1 proteins on the paraffin embedded sections from 20 active tuberculosis lymphadenitis,20 obsolete foci and 20 chronic nospecific lymphadenitis.The immunohistochemical results were analyzed quantitatively using an image analysis system.Results Multinucleate giant cells as well as histocytes in granuloma and macrophages around the necrosis areas in active tuberculosis lymphadenitis were all strongly positive for MMP-9,while in other group MMP-9 were weakly positive.The expressions of MMP-9,TIMP-1,and MMP-9/TIMP-1 in active tuberculosis group were significantly higher than those in the chronic nonspecific lymphadenitis group and the obsolete foci group (P<0.01).No significant difference was observed between simple active tuberculosis lymphadenitis and foci complicated with active pulmonary tuberculosis.Conclusion The expression level of MMP-9 and TIMP-1 protein may play an important role in tuberculosis destruction mechanisms and the higher ratio of MMP-9/TIMP-1 (the imbalance between MMPs and TIMPs) may indicate a higher risk of progression of disease and dissemination of tuberculosis.更多还原