【摘要】 目的:探讨白介素(IL)-4受体在系统性红斑狼疮(SLE)发病中的作用及其临床意义。方法:应用逆转录-聚合酶链反应(RT-PCR)检测89例SLE患者和30名健康对照者外周血单一核细胞(PBMC)中IL-4受体mRNA的表达水平;用酶联免疫吸附试验(ELISA)检测血清中IL-4水平。结果:①活动期SLE患者、非活动期SLE患者及健康对照者PBMCIL-4受体表达阳性率为100%。②活动期SLE患者、非活动期SLE患者及健康对照者PBMCIL-4受体mRNA表达水平分别为0.604±0.147、0.400±0.130、0.370±0.070。活动期SLE患者和非活动期SLE患者比较、活动期SLE患者和正常对照者比较差异均有显著性(P=0.000),非活动期SLE患者和正常对照者比较差异无显著性(P=0.351)。③血清中IL-4水平在活动期SLE患者显著高于非活动期SLE患者和正常对照组(P=0.000),非活动期SLE患者显著高于正常对照组(P=0.000),活动期和非活动期SLE患者血清中IL-4水平与PBMCIL-4受体表达水平呈正相关(r=0.622～0.859,P<0.05)。结论:IL-4及其受体的异常表达可能在SLE疾病活动和进展过程中起重要作用。检测SLE患者PBMCIL-4受体的表达水平可作为了解疾病活动性的指标之一。更多还原

【Abstract】 Objective: To investigate the roles of IL-4 and IL-4R in the pathogenesis of systemic lupus erythematosus (SLE) and their clinical significance. Methods: RT-PCR technique was used to semiquantitatively analyze IL-4R mRNA expression in PBMC, and ELISA was applied to determine IL-4 in the serum from 89 patients with SLE. Results: The positive IL-4R mRNA expression was detected in each individual among active SLE group, inactive SLE group and the controls. The mean levels of IL-4R mRNA expression in active SLE group, inactive SLE group and control group were 0.604±0.147, 0.400±0.130 and 0.370±0.070, respectively. Compared with inactive SLE group and control group, the mean level of IL-4R mRNA expression in active SLE group increased significantly(P = 0.00), while there was no statistical differences between inactive SLE group and control group (P = 0.351). The mean levels of IL-4 in serum in active SLE group, inactive SLE group and control group were (105.570±29.170) pg/mL, (51.170±16.690)pg/mL and(23.730±6.240)pg/mL, respectively. Compared with inactive SLE group and control group, the mean level of IL-4 in active SLE group was significantly higher than that in inactive SLE group and control group (P = 0.000), while that in inactive SLE group was higher than control group (P = 0.000). There was positive correlation between IL-4 and IL-4R in active and inactive groups(r = 0.622~0.859, P < 0.05). Conclusions: The expression of IL-4R mRNA in PBMC and the levels of IL-4 in the serum of patients with active SLE were significantly higher than those in patients with inactive SLE and control group. The mean level of IL-4 in patients with inactive SLE was higher than that in the controls. The abnormal expression of IL-4 and IL-4R might play an important role in the pathogenesis of SLE, and the level of IL-4R might be used as an index for active SLE.更多还原