【摘要】 以一株从腐朽竹子上分离得到的侧耳菌(Pleurotus sp．)GH196为产酶菌,研究了其 在静置和振荡两种液体培养方式下的木质纤维素降解酶系的产生。结果表明:在含有稻草和 麦麸的液体培养基中生长,可以产生木聚糖酶、纤维素酶和依赖锰过氧化物酶-漆酶型(MnP- Laccas型)木质素降解酶。振荡条件可以促进菌丝生长,有利于多糖水解酶的产生,其中以木聚糖酶的合成为 主;150 r／min振荡培养5 d,木聚糖酶酶活达到44．7 U／mL。更多还原

【Abstract】 To investigate the production of lignocellulose-degrading enzyme system from Pleurotus sp.GH196,isolated from a rotten bamboo,submerged fermentation of this fungus was performed under shaking and static conditions.The result showed that lignolytic enzymes produced by GH196 are composed of laccase and manganese peroxidase. Shaking could promote the mycelium growth of the fungus and synthesis of cellulase and xylanase,among them xylanase is the master polysaccharide hydrolase.The xylanase activity can reach 44.7 U/mL when GH196 was cultivated by using rice straw and wheat bran as compound carbon source under 150 r/min shaking condition.更多还原