【摘要】 目的:探讨TPA对体外培养的人喉鳞状细胞癌细胞株HEp-2细胞的作用。方法:应用不同浓度TPA处理体外培养的HEp-2细胞,MTT法检测HEp-2细胞的存活比率。结果:HEp-2细胞经TPA处理后其细胞增殖能力呈现峰型,高峰出现在10μg/L水平上,此时处理细胞的增殖能力是对照组的1.3~1.4倍。随着TPA浓度的增高,HEp-2细胞的增殖能力逐渐下降。结论:TPA处理可以增强HEp-2细胞的增殖能力。更多还原

【Abstract】 Objective:To investigate the influence of TPA treatment on HEp-2 cell line in vitro.Method:HEp-2 cell line cultured in vitro was treated by different concentration levels of TPA. MTT method was used to detect survival cell ratio of HEp-2. Result:Cell growth of HEp-2 after treatment of TPA manifested an peak.The highest peak which was 1.3 to 1.4 times of control cell presented on 10 μg/L concentration level.Cell proliferation ability dropped gradually following increase of TPA concentration.Conclusions:TPA can increase cell proliferation of HEp-2 cell in vitro.更多还原