【摘要】 目的:研究尿激酶型纤溶酶原激活剂(uPA)及其特异受体(uPAR)在喉癌组织中的表达及临床意义。方法:应用免疫组织化学SABC法分别检测uPA、uPAR在50例喉癌和10例对照组中蛋白水平的表达。Spearman法和MannWhitneyU法分析它们表达的相关性及与喉癌临床病理参数的关系,χ2检验分析其与颈淋巴结转移的关系。结果:喉癌组织中uPA和uPAR阳性表达率分别为66%和62%,且表达呈正相关(P<0.05)。uPA和uPAR的表达水平与临床分期、颈淋巴结状况相关,与肿瘤大小、T分期、病理学分级无关。uPA和uPAR同时阳性表达组颈淋巴结转移率明显高于同时阴性表达组,两组间比较差异有统计学意义(P<0.01)。结论:uPA和uPAR表达在喉癌中明显升高,uPA和uPAR与喉癌的浸润转移密切相关。更多还原

【Abstract】 Objective:To study the expressions and cli ni cal significance of urokinase-type plasminogen activator (uPA) and uPA receptor (uPAR) in laryngeal squamous cell carcinoma (LSCC).Method: The expressions of uPA and uPAR at protein level were examined in 50 cases of LSCC and 10 cases of normal peripheral tissues around cancer (as control) by str ep avidin-biotin complex (SABC) immunohistochemical technique. The relationship between the expressions of uPA and uPAR was evaluated by the Spearman’s rank co rrelation test. Mann-Whitney U-test was used to examine association of these f actors with conventional clinical pathological factors.Pearson’s chi-square tes t was used to analyze the relationship between uPA, uPAR and cervical lymph node status. Result:The rates of the positive expression in LS CC of uPA and uPAR were 66% and 62% respectively, and a positive relationship be tween expression of uPA and uPAR(P<0.05). There was significant correlatio n between the level of uPA expression and TNM stage or cervical lymph node statu s, either between the level of uPAR expression and TNM stage or cervical lymph n ode status. Neither tumor size,T-category, nor grade was significantly related with the level of uPA or uPAR expression. LSCC cases with concourse positive exp ression of these proteins revealed significant higher tendency of cervical lymph node metastasis than those with concourse negative expression (P<0.01). Conclusion:The expression of uPA and uPAR are increased in L SCC, and uPA,uPAR may contribute significantly to LSCC invasion and metastasis.更多还原