【摘要】 目的:探讨血小板源性生长因子-B(PDGF-B)基因转染修饰大鼠骨髓间充质干细胞(bone m arrow m esenchym alstem cell,M SC)的可行性。方法:应用FAM标记重组真核表达载体系统(pcDNA3-PDGF-B),以脂质体法转染原代骨髓间充质干细胞,观察转染结果、表达情况及对靶细胞活力的影响。结果:经荧光显微镜下观察证实:成功地对骨髓间充质干细胞实现了基因转染,持续表达时间超过了8周,没有发现明显的细胞毒作用及对细胞活力的显著影响。结论:采用真核转染技术可以介导外源基因转染骨髓间充质干细胞,M SC是一种理想的基因载体细胞,可用于PDGF-B的基因治疗。更多还原

【Abstract】 Objective:To study the feasibility of the Platelet derived growth factor-B（PDGF-B） gene transfection to the bone marrow mesenchymal stem cell（MSC）.Methods:The MSC obtained from bone marrow of adult Wistar rat were cultured,and treated with FAM-marked recombinant pcDNA₃-PDGF-B,and transfected by liposome method.The transgene was confirmed by fluoroscopy and the post-transfection cell viability was evaluated by light microscopy and electron microscopy.Results:Bone marrow MSC was successfully transfected with pcDNA₃ carrying PDGF-B gene and the exogenous gene expression lasted over 8 weeks.The cell viability was not significantly influenced and no cell toxicity was observed.Conclusion:Bone marrow MSC can be efficiently modified by appropriate gene transfection through recombinant technique.MSC is ideal cells for cell-mediatedPDGF-B gene therapy or cell therapy.更多还原