【摘要】 目的:探讨TNF鄄α基因转导的人舌癌Tca8113细胞在体外能否诱导口腔鳞癌的引流区淋巴结细胞(DNL)而提高起其细胞毒活性。方法:体外用逆转录病毒载体将TNF鄄α基因导入Tca8113细胞并进行放射灭活,灭活的转基因细胞与来自舌鳞癌患者的DNL共培养后,采用LDH释放改良法检测诱导后DNL对未转基因Tca8113细胞的杀伤活性。结果:转基因Tca8113细胞诱导的DNL对未转基因Tca8113细胞的杀伤活性高于未经诱导的DNL。结论:TNF鄄α基因修饰的Tca8113细胞能够从肿瘤引流区淋巴结细胞中诱导出高细胞毒活性的免疫活性细胞,诱导出的免疫细胞中可能含有CTL。更多还原

【Abstract】 Objective: To investigate if cytotoxicity of DNL can be enhanced in vitro by inducement of TNF-α gene-transduced human Tca8113 cells. Methods: The TNF-α gene was transduced into the Tca8113 cells, and the transgenic cells were inactivated by irradiation. After the irradiated transgenic Tca8113 cells were cultured with the DNL obtained from a patient with squamous cell carcinoma of tongue, antitumor effect of the DNL against non-transgenic Tca8113 was analyzed by improved LDH release assay. Results: The DNL induced by TNF-α gene-transduced Tca8113 cells showed higher cytotoxicity than the parental DNL did. Conclusion: Cytotoxicity of DNL can be enhanced in vitro by inducement of TNF-α gene-transduced human Tca8113 cells, and it is supposed that there are some CTL in induced DNL.更多还原