【摘要】 目的:研究雌激素对大鼠骨髓基质细胞增殖和分化的影响,探讨其对成骨的影响。方法:体外培养4周龄雌性大鼠骨髓基质细胞,以不同浓度雌激素作用于成骨细胞,用噻唑蓝法测定细胞增殖情况;对硝基酚磷酸酯法测定细胞碱性磷酸酶活性;检测培养液中羟脯氨酸含量,了解细胞I型胶原的分泌情况;通过矿化结节计数反映细胞的矿化能力。结果:雌激素促进成骨细胞增殖,增加碱性磷酸酶活性;提高培养液中羟脯氨酸含量,峰值浓度为10-7mol/l,与对照组比较有统计学意义(P<0.05);雌激素显著提高细胞的矿化能力,10-7mol/l浓度作用最显著(+45.4%,P<0.05)。结论:雌激素促进成骨细胞增殖和分化,提高其矿化能力,从而刺激骨形成。更多还原

【Abstract】 Objective:To study the effect of estrogen on proliferation and differentiation of rat bone marrow stromal cells and to explore the mechanism of stimulation of bone formation by estrogen. Methods:Bone marrow stromal cells were isolated from four-week-old female Sprague-Dawley rats and cultured in DMEM medium to be induced into osteoblasts. They were cultured in medium with estrogen at different concentrations. The prolifereation, ALP activity and type-I pro-collagen of osteoblasts were examined by MTT, PNPP and assaying the level of hydroxyproline. The capacity of mineralization were measured by counting bone nodules. Results:The cell quantity, the alkaline phosphatase activity and the level of hydroxyproline of osteoblasts were increased , and the number of nodules was significantly higher. The peak value of all these stimulating effects on osteoblasts was in 10-7mol/L group. Conclusion:Estrogen can increase proliferation, differentiation and the capability of mineralization of osteoblast and this may contribute to bone formation.更多还原