【摘要】 目的了解儿童福氏志贺菌是否存在TEM基因及其发生率以及对耐药性的影响。方法对2004年6—11月从本院细菌性腹泻患儿粪便培养标本分离到的福氏志贺氏菌共51株,进行β内酰胺酶TEM全长基因PCR检测;用K-B法测定志贺菌对7种抗菌药物的敏感度。结果51株福氏志贺菌中17株检出TEM基因,阳性率33.3%;志贺菌对氨苄西林的耐药率高,其中TEM基因阳性志贺菌的耐药率为88.2%、TEM基因阴性志贺菌的耐药率为82.4%;对氨苄西林-舒巴坦也有一定的耐药,TEM基因阳性志贺菌、TEM基因阴性志贺菌的耐药率分别为41.2%、20.6%。结论从福氏志贺菌检出TEM型β内酰胺酶耐药基因,携带率为33.3%。更多还原

【Abstract】 ObjectiveTo investigate the TEM-type βlactamase gene in Shigella flexneri and its prevalence in children,as well as the impact of the gene on bacterial resistance.MethodsFifty-one strains of S.flexneri were isolated from faecal specimens of children with bacterial diarrhoea between June 2004 and November 2004 at Children′s Hospital of Fudan University.TEM-type β-lactamase gene was detected by polymerase chain reaction(PCR).The susceptibility of S.flexneri strains to 7 antimicrobial agents was determined by K-B method.ResultsTEM-type β-lactmase gene was detected in 17(33.3%,17/51) strains of S.flexneri.The S.flexneri isolates were highly resistant to ampicillin.The resistance rate of TEM-gene positive S.flexneri was 88.2%,and that of TEM-gene negative S.flexneri was 82.4%.About 41.2% of TEM-gene positive S.flexneri and 20.6% of TEM-gene negative S.flexneri were resistant to ampicillin-sulbactam.ConclusionsTEM gene was detected in(33.3)% of S.flexneri isolates from children.更多还原