【摘要】 目的研究基质金属蛋白酶9基因C1562T多态性与中国汉族人群心肌梗死易感性的关系。方法以78例经冠状动脉造影确诊的心肌梗死患者为研究对象,以同期冠状动脉造影阴性、排除冠心病诊断的81例患者为对照组,取外周血标本提取DNA,用聚合酶链反应方法扩增目的基因,用限制性内切酶酶切聚合酶链反应产物用于基因分型。比较两组间基质金属蛋白酶9基因多态性频率分布的差异。结果本研究中未发现基质金属蛋白酶9的TT基因型突变。心肌梗死患者基质金属蛋白酶9基因CT基因型频率(26.9%)高于对照组(9.9%),两组差别有统计学意义(χ2=7.743,P=0.005),心肌梗死组1562T等位基因频率(13.5%)高于对照组(4.9%),两组差别也有统计学意义(χ2=6.966,P=0.008)。结论基质金属蛋白酶9基因C1562T多态性与中国汉族人群心肌梗死有关,1562T等位基因可能是心肌梗死遗传易感性的基因标记之一。更多还原

【Abstract】 Aim To investigate the association between myocardial infaction(MI) and functional matrix metalloproteinase-9 polymorphism(C1562T). Methods A case-control study of seventy-eight patients with angiographically documented MI and eighty-one control subjects with a normal angiogram was conducted.Genotype was determined by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) for the common C1562T functional promoter polymorphism of the MMP-9 gene. Results The results of individual polymorphisms analysis showed that the frequency of C/T genotype and 1562T allele of MMP-9 gene of MI patients(26.9% and 13.5%) were significantly higher than that in control group(9.9% and 4.9%;P<0.01). Conclusion The present findings suggest that the genetic polymorphism in MMP-9 promoter(C1562T) is associated with the susceptibility to MI in the Han population of China.更多还原