【摘要】 目的观察槲皮素、异鼠李素和去甲肾上腺素对人血管平滑肌细胞胶原蛋白合成的影响。方法利用培养的人主动脉血管平滑肌细胞,在培养基中加入不同浓度的槲皮素、异鼠李素、去甲肾上腺素及酚妥拉明,孵育不同时间。采用3H脯氨酸掺入法检测胶原蛋白的合成量。结果10μmol/L去甲肾上腺素有促进血管平滑肌细胞胶原蛋白合成的作用,72h达高峰,去甲肾上腺素的促进作用能被酚妥拉明所阻滞。槲皮素和异鼠李素有抑制血管平滑肌细胞胶原蛋白合成的作用,在1～200μmol/L的浓度范围内其抑制作用有剂量依赖关系,200μmol/L浓度时发挥最大的抑制作用。槲皮素和异鼠李素均对去甲肾上腺素促血管平滑肌细胞胶原蛋白合成有明显的剂量依赖抑制效应,72h抑制作用最强。槲皮素和异鼠李素在抑制去甲肾上腺素的刺激作用方面具有明显的协同作用。槲皮素和异鼠李素对去甲肾上腺素促血管平滑肌细胞胶原蛋白合成的抑制作用,明显大于未受去甲肾上腺素作用的血管平滑肌细胞。槲皮素和异鼠李素对去甲肾上腺素刺激作用的抑制也明显强于酚妥拉明的作用。结论槲皮素和异鼠李素对人主动脉血管平滑肌细胞的胶原蛋白合成,尤其是对去甲肾上腺素刺激的血管平滑肌细胞胶原蛋白合成有很强的抑制作用。更多还原

【Abstract】 Aim To investigate the effects of quercetin (QUE), isorhamnetin (ISOR) and norepinephrine (NE) on collagen synthesis of cultured human aortic vascular smooth muscle cell (VSMC). Methods Human aorta VSMC was cultured with QUE, ISOR, NE, phentolamine(Ph). Collagen synthesis was evaluated by measuring 3 H-proline incorporation. Results NE 10 μmol/L could stimulate collagen synthesis of VSMC, and the most effects of the stimulation occurred at 72 h. The effects can be inhibited by Ph. QUE and ISOR could inhibited collagen synthesis of VSMC. When VSMC were treated by 1～200 μmol/L of QUE or ISOR, the best inhibitory effects occurred at 200 μmol/L. There were dose-dependent inhibitory effects in different concentration of QUE and ISOR and the best effect of QUE occurred at 72 h. QUE and ISOR markedly inhibit collagen synthesis of VSMC induced by NE in a dose-dependent manner and the best effects occurred in 72 h after treated with QUE or ISOR. At the same time QUE and ISOR had cooperative effects on inhibition of the stimulation of NE. QUE and ISOR had more powerful inhibitory effect on collagen synthesis of VSMC co-incubation with NE than without NE. QUE and ISOR also had more powerful inhibitory effect on collagen synthesis of VSMC co-incubation with NE than Ph. Conclusions QUE and ISOR could effectively inhibit the collagen synthesis of VSMC, especially inhibit the collagen synthesis of VSMC stimulated by NE.更多还原