【摘要】 目的研究单核细胞趋化蛋白1对人脐静脉平滑肌细胞增殖的影响。方法用不同浓度单核细胞趋化蛋1(0.1、1.0、10及100μg/L)作用于人脐静脉平滑肌细胞,采用细胞计数、MTT、流式细胞术检测细胞增殖情况;采用逆转录聚合酶链反应检测细胞中cfos基因的表达。结果单核细胞趋化蛋白1能诱导人脐静脉平滑肌细胞的增殖,呈剂量依赖关系,100μg/L单核细胞趋化蛋白1对人脐静脉血管平滑肌细胞的增殖作用强于平滑肌细胞增殖因子血小板源生长因子的作用(P<0.001),单核细胞趋化蛋白1在诱导血管平滑肌细胞增殖的同时引起cfos基因的高表达(P<0.001)。结论单核细胞趋化蛋白1不仅是趋化激活剂,而且是人脐静脉平滑肌细胞的促增殖因子,并且其促增殖的功能可能与增强c-fos基因的表达有关。更多还原

【Abstract】 Aim To study the effect of monocyte chemotactic protein-1 (MCP-1) on the proliferation of human umbilical vascular smooth muscle cells (hUVSMC). Methods hUVSMC were cultured in vitro. Growth-arrested hUVSMC were stimulated with different concentrations of MCP-1 (0.1, 1.0, 10 and 100 μg/L). The response of hUVSMC to these treatments was observed in comparison with that of platelete derived growth factor (PDGF). The proliferation of hUVSMC was evaluated by cell counting, MTT assay and flow cytometry. The expression of c-fos mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Results MCP-1 stimulated the proliferation of hUVSMC in a dose-dependent-manner. Compared with PDGF, the effect of MCP-1 at high concentration was significant (P<0.01). Meanwhile, MCP-1 induced the expression of c-fos mRNA. Conclusions MCP-1 could promote the proliferation of hUVSMC and the expression of c-fos mRNA. The effect of MCP-1 at high concentration was more significant than that of PDGF. MCP-1 may exert its effects on cells through c-fos.更多还原