【摘要】 采用经典的CO差光谱方法测定棉铃虫Helicoverpa armigera细胞色素P450含量时发现, 同一样品的多次平行测定结果间差异显著。进一步研究表明测定时通入CO后的间隔时间不同, 计算出的P450含量存在1~16倍的差异。多次连续扫描结果表明A450nm-490nm与通入CO后到扫描的时间间隔呈钟形曲线, 说明CO与P450的结合需要一定的时间, 以A450nm-490nm最大值计算P450含量最为准确。更多还原

【Abstract】 The CO difference spectra of cytochrome P450 (P450) in midgut and fat body of cotton bollworm, Helicoverpa armigera (Hübner), were investigated. The experiment indicated with traditional methods for quantifying cytochrome P450, the result was not easy to repeat even to the same sample. The interval between CO addition and measurement greatly influenced the value of A (450nm-490nm). There was one to sixteen fold differences in optical density. In repetitive analysis on CO difference spectra of P450 in midgut and fat body, it was found that the optical density difference between 450 nm and 490 nm increased within a period of time and then decreased gradually. It was concluded that the parameter of the maximum optical density difference between 450 nm and 490 nm was most accurate in determining the content of P450.更多还原