【摘要】 本文用7个绿僵菌(Metarhizium)菌株对德国小蠊Blattella germanica(Linnaeus) 进行注射生测,筛选出了一个致病菌株进行五代复壮选育。该株绿僵菌Metarhizitun anisopliae (Metsch)Sorokin复壮前对雌雄成虫注射生测LD50依次为13.2与8.9天;复壮后对雌雄成虫背部点滴生测12天的LDm值均为1.3×10~5孢子/虫。通过CP包埋剂制作石蜡切片对僵虫体内菌丝核和体表孢子堆进行了观察。更多还原

【Abstract】 The virulence of 7 Metarhizium strains against the German cockroaches Blattella germanica (Linnaeus) was selected by injection method, and a pathogenic strain Metarhizium anisopliae (Metsch) Sorokin was passed through five times rejuvenation. The LT50 values of the bioassay by injection method were 13.2d (female) and 8.9d (male) before the rejuvenation; the LD50 (12 day) was 1.3×105 spores per cockroach after the rejuvenation. Using CP embedding tissue section technique, the microstructure of the muscardine cadavers’ sori and mycelial mass was observed.更多还原