【摘要】 目的观察果糖二磷酸镁(FDP-M)对心肌缺血-再灌注损伤过程中血清中CK,LDH,Mg2+浓度、心肌组织内SOD,MDA浓度的影响,探讨果糖二磷酸镁心肌保护作用的机理及其剂量依赖型。方法用整体大鼠急性心肌缺血再灌注损伤模型,结扎冠脉左室前降支40min后,再灌注40min,应用比色法测定血清中CK,LDH,Mg2+含量,采用羟胺法测定心肌组织中SOD含量,采用硫代巴比妥钠(TAB)比色法测定心肌组织内MDA含量。结果与假手术组相比,缺血再灌注损伤组血中CK,LDH浓度显著升高,Mg2+浓度显著降低,组织内MDA含量明显升高,SOD含量明显降低。与缺血损伤组相比,果糖二磷酸镁三个不同剂量保护组血中CK,LDH浓度明显降低,Mg2+浓度明显升高,组织内MDA含量明显降低,SOD含量明显升高,差异具有显著性(P<0.05或P<0.01),此作用均可见明显剂量依赖性。结论果糖二磷酸镁对心肌缺血再灌注损伤有保护作用,并呈明显的剂量依赖型,其保护作用机制与增加组织内SOD含量,减少血中CK,LDH浓度,减少组织内MDA含量有关。更多还原

【Abstract】 Objective This paper was designed to observe the concentration change of CK,LDH,Mg²⁺ in the blood serum and SOD,MDA in the myocardial to investigate the cardioprotective effect of Magnesium Fructose-1,6-diphosphate during myocardial ischemia reperfusion injury in anesthetized rats.Methods We adopted the model of myocardial ischemia reperfusion injury in anesthetized rats,determined the concentration of CK,LDH and Mg²⁺ in blood serum,and we determined the contents of SOD and MDA in myocardium in the same time.Results The concentration of Mg²⁺ and SOD were decreased and the concentration of CK,LDH and MDA were increased in the ischemia reperfusion group compared with the sham,and concentration of Mg²⁺ and SOD were significantly increased and CK,LDH,MDA contents were decreased in the three groups of Magnesium Fructose-1,6-diphosphate compared with the ischemia reperfusion group.The dosage dependence was observed of the effect mentioned above.Conclusions Magnesium Fructose-1,6-diphosphate has protective effects on myocardial ischemia reperfusion injury.The protective effect may be related to increasing concentration of Mg²⁺ and SOD,relieving CK,LDH,MDA and antilipid peroxidation.更多还原