【摘要】 目的观察宣肺透解剂对流感病毒感染小鼠肺病毒滴度及辅助性T细胞1型(Th1)细胞因子的作用,研究其抗病毒作用的可能机制。方法建立流感病毒鼠肺适应株(FM1)感染的小鼠肺炎模型,用微量血凝实验检测小鼠肺组织中病毒滴度,用生物活性测定法检测小鼠血清中白细胞介素-2(IL-2)、γ-干扰素(IFN-γ)的活性水平。结果宣肺透解剂可降低感染小鼠肺组织病毒滴度,同时提高血清中IL-2、IFN-γ的活性水平。结论宣肺透解剂可能通过调节Th1型细胞因子的活性而增强细胞毒T细胞(CTL细胞)、自然杀伤细胞(NK细胞)和巨噬细胞对病毒的清除。更多还原

【Abstract】 Objective To observe the effect of Xuanfeitoujie Formula (XF) on viral titer and Th1 cytokines in mouse lung infected by influenzavirus, and to investigate the mechanism of antiviral effect of XF. Method To establish the mouse model of pneumonitis induced by FM1 adaptive strain of influenzavirus and detect the viral titer in mouse lung by hemagglutinative test. The bioactivity of interleuking-2 (IL-2), interferon-γ (IFN-γ) in mouse serum was detected by bioactive method. Result XF decreased the viral titer in mouse lung and increased the bioactivity of IL-2 and IFN-γ in mouse serum. Conclusion XF can help cytotoxic lymphocytes (CTL), NK cells and macrophages to clear virus through regulating the activities of Th1 cytokines.更多还原