【摘要】 目的观察针灸大椎穴对慢性应激失调大鼠行为学及下丘脑室旁核精氨酸加压素(AVP)阳性神经元数量的影响。方法按随机数字表随机将SD雄性大鼠分为正常组、模型组、艾灸组、针刺组。应用分养和长期不可预见性的中等强度刺激造成慢性应激失调模型,观察各组大鼠精神状态、体重、蔗糖偏嗜度、旷场实验等行为学的不同;用免疫组织化学和图像分析法比较各组动物下丘脑室旁核精氨酸加压素阳性神经元的数量。结果慢性应激可致大鼠活动明显减少、食欲不振、精神萎靡,并且体重增加数、蔗糖偏嗜度、旷场实验中活动次数显著减少;下丘脑室旁核AVP阳性神经元的数量明显高于正常组。艾灸或针刺大椎穴均可明显改善上述情形、下调AVP阳性神经元的数量(P<0.05~P<0.01)。艾灸与针刺比较无明显差异。结论艾灸与针刺大椎均可明显改善抑郁大鼠的行为异常,其作用可能是通过对应激中枢下丘脑AVP的调节实现的,二者无明显差异。更多还原

【Abstract】 Objective To observe the effect of moxibustion or acupuncture in Dazhui(GV14) acupoint on behavior and positive neuron numbers of arginine vasopressin(AVP) in nucleus paraventricularis hypothalami(NPH) in rats with chronic stress disturbance. Methods To divide randomly SD rats into the normal group,model group,moxibustion group and acupuncture group according to the Random Number Table. The model of chronic stress disturbance was established through single housed and long-term mild unpredictable stimulation.Some ethology indexes,such as mental status,weight,sucrotropic degree,wide-field test result were observed in different groups.The numbers of positive neuron of AVP in NPH in different groups were compared by using immunohistochemistry and imag analysis.Results The chronic stimulation resulted in the decrease of rat activity,inappetence,dispiritedness,weight,sucrotropic degree,and activity numbers in wide-field test.The positive neuron numbers of AVP in NPH in the model group was higher obviously that that of the normal group.In the moxibustion and acupuncture groups the above behaviors of rats were relieved and the positive neuron numbers of AVP were decreased(P<0.05-P<0.01).There was no significant difference between the moxibustion group and acupuncture group.Conclusion Moxibustion or acupuncture in Dazhui(GV14) acupoint all can improve obviously the behaviors of depressed rats.The mechanism maybe related to the regulation of AVP in NPH by moxibustion or acupuncture.Moxibustion differed insignificantly from acupuncture.更多还原