【摘要】 目的:观察钌红染色方法标记大鼠特发性肺间质纤维化(id iopath ic pu lmonary fibrosis,IPF)的超微结构的特点。方法:180只SD大鼠随机分为6组,盐酸博莱霉素气管内注射造模,复方鳖甲片高、中、低剂量治疗。分别于7d、14d、28d取材,钌红染色电镜观察。结果:钌红明显标记基底膜和表浅层细胞周围的胶原纤维。使细胞轮廓和内部结构更清晰。7d纵断面既可显示成束的胶原纤维,特别是基膜和细胞之间有成束的胶原纤维堆积。14d上皮细胞外基膜区域性折叠、扭曲或缺损。基板处钌红沉积,呼吸膜增厚。28d基膜厚薄不均匀,完整性破坏。Ⅱ型细胞在三阶段不同病理时期有不同改变。细胞内前胶原增多,粗面内质网扩张,胶原纤维在间质大量堆积,排列纵横交错。结论:钌红染色更清晰显示基底膜和表浅层细胞周围的胶原纤维,更利于观察IPF的纤维分布和厚度。更多还原

【Abstract】 Objective:To investigate ultrastructure characteristic in the ruthenium red staining on the rats with IPF.(Methods:180) male SD rats were randomly divided into six groups:making a model by injecting BLM in trachea,treating by high-dosage,medium-dosage and low-dosage medicine.each group are killed respectively on 7th day,14th day and 28th day.the ultrastructural change by ruthenium red staining were observed.Results:Collagen fiber is in focus around basement menbrane and cell.Cell menbrane and organelle is more clear,there are pathological change of AT-Ⅱ on 7th day,14th day and 28th day in differ phase.we can see a binds of collagen fiber espesially between basement menbrane and cell.14th day basement menbrane outboard there are pucker and distortion or in faith.There are ruthenium red staining sediment in the basement menbrane,blood-air barrier becomes thicker.28th day basement menbrane becomes asymmetry and integrality is destroyed.Forecollagen fiber are monifolding and rough endoplasmic reticulums in the cell are expanding,collagen fiber accumulates in interstitial and arrange cross.(Conclusion:Basement) menbrane and collagen fiber round cell may be revealed more clear in the ruthenium red staining on the rats with IPF.it is more convenience observing distributing of collagen fiber and thick of basement menbrane.staining;idiopathic pulmonary fibrosis;collagen;microtomy更多还原