【摘要】 目的:观察褪黑激素(MT)对吗啡成瘾大鼠红细胞变形能力(ED)的影响并对其机制进行初步探讨。方法:给大鼠连续递增皮下注射吗啡5d,建立吗啡成瘾模型,给实验组大鼠腹腔注射MT(50mg/kg)后,用纳络酮催瘾(0.5mg/kg);另外,向正常大鼠血中分别加入去甲肾上腺素、三磷酸腺苷、吗啡、MT,检测大鼠ED。结果:成瘾组较对照组ED明显下降(P<0.01),MT实验组较成瘾组ED明显升高(P<0.01);去甲肾上腺素、三磷酸腺苷、吗啡、使正常大鼠ED不同程度的下降,而MT使ED升高,并呈一定的剂量效应关系。结论:MT能明显改善吗啡成瘾大鼠ED下降。这可能是通过MT对RBC的直接保护作用,也可能是抑制由戒断引起交感神经系统和G蛋白cAMP系统亢进而发生的。更多还原

【Abstract】 Objective:Studying the effect and mechanism of MT on erythrocyte deformability in morphine dependent rats. Methods:After 5 days, a model of morphine dependent rats was established by gradually increasing the doses of morphine subcutaneous injection for rats. MT test group rats was injected with MT(50mg/kg) by peritoneoscope, Meanwhile, Then rats were injected with naloxone (0.5mg/kg).ED was detected to observe the changes of ED of rats. Results:ED of morphine dependent rats was lower than that of the normal control. MT could obviously inhibit the above change. Moreover, if morphine, NE, cAMP of different doses were directly added into blood of normal rats ,it could reduce the ED clearly; But MT could have an betterment to the change in a dose dependent manner. Conclusion:MT may obviously improve the decreasing of ED in morphine dependent rats. It may be resulted by protecting RBC directly or inhibiting the hyperfunction of the sympathetic system and protein G-cAMP system.更多还原