【摘要】 目的建立实时PCR技术快速检测宫颈组织HPV16感染。方法根据GeneBank中HPV16E6序列,设计合成特异的引物和MGB荧光探针,优化实验条件,并对40例宫颈癌,38例宫颈非典型增生,20例正常对照宫颈组织标本进行HPV16DNA检测。结果建立了灵敏、特异地检测宫颈组织HPV16DNA的实时PCR技术;宫颈癌、宫颈非典型增生和正常对照宫颈组织中HPV16DNA阳性率分别为42.5%、34.2%和0%;宫颈癌组织中HPV16DNA平均拷贝数(106.01±1.22拷贝/mL)高于宫颈非典型增生组(105.78±1.37拷贝/ml),但差异无显著性(P>0.05)。结论实时PCR检测宫颈组织内HPV16感染,具有快速、简便、特异性强等优点,适用于临床常规检测。更多还原

【Abstract】 Objective To establish a rapid assay for detecting infection of human papillomavirus (HPV) type16 by real-time PCR. Methods According to HPV16 E6 sequence,the primers and MGB probes targeted were designed and applied to quantify HPV16 DNA by real- time PCR in 40 cases of cervical cancer, 38 cases of cervical dysplasia and 20 healthy persons as control. Results A sensitive and specific real-time PCR assay for HPV16 detection was set up. The positive rates of HPV16 DNA was 42.5%, 34.2% and 0% in tissues of cervical cancer, cervical dysplasia and the controls respectively. The normal copies of HPV16 DNA were not significantly different between cervical cancer (106.01±1.22copies/ml) and cervical dysplasia(105.78±1.37copies/ml). Conclusion The detection of HPV16 DNA by real-time PCR based on Taqman-MGB technique is more rapid,simple,and specific,and it is suitable use in clinical laboratory.更多还原