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β-D-葡萄糖苷酶对桂花香气成分的影响

Effect of β-D-Glucosidase on Aromatic Compounds of Sweet Osmanthus

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【作者】 杨志萍姚卫蓉钱和

【Author】 YANG Zhi-ping,YAO Wei-rong~,QIAN He(School of Food Science and Technology,Southern Yangtze University,Wuxi 214036,Jiangsu,China)

【机构】 江南大学食品学院教育部功能食品工程研究中心江南大学食品学院教育部功能食品工程研究中心 江苏无锡214036江苏无锡214036

【摘要】 用β-D-葡萄糖苷酶酶解桂花鲜花后,利用固相微萃取法(SPME)分别分离酶解前后桂花香气成分,再用气相色谱-质谱联用仪(GC/MS)分析鉴定,并通过其总离子流色谱峰的峰面积进行归一化定量。经与酶解前的26种主要香气成分分析比较发现,酶解后,桂花主要香气成分质量分数均明显增长,其中顺、反-氧化芳樟醇,L-芳樟醇,萜烯二醇,L-α-萜品醇等的增长率分别达到176.4%、262%、113.5%、102.6%和189.8%。这充分证明了β-D-葡萄糖苷酶可水解鲜花香气的前体物质,释放出鲜花中潜在的香气成分,从而大幅度提高香气成分的质量分数。

【Abstract】 The aromatic compounds of sweet osmanthus were investigated by GC/MS.The volatiles before and after enzyme hydrolysis were sampled by solid-phase microextraction(SPME).According to SPME-GC/MS analysis,after enzyme hydrolysis,the mass fraction of main aromatic compounds increased clearly,especially cis-linalool oxide,trans-linalool oxide,L-linalool,terpendiol and L-α-terpineol,the increasing ratios of which reached 176.4%,262%,113.5%,102.6% and 189.8% respectively,compared with the sample before β-D-glucosidase hydrolysis.It was found that β-D-glucosidase could release the potential aromatic compounds of fresh flower after hydrolyzing the flavor precursors,as a result,it enhanced the mass fractions of the aromatic compounds.

  • 【分类号】TQ651
  • 【被引频次】31
  • 【下载频次】426
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