【摘要】 从黑麦草Lolium perenne5个栽培品种中分离、纯化得到6株形态稳定的内生真菌,它们在常规培养条件下均不产生分生孢子。通过在PDA和MEA培养基上进行预培养、扫刷营养体、近紫外光照射以及在菌丝体上放置宿主植物等诱导方法,使其中5株真菌不同程度产生了分生孢子,通过分生孢子的形态成功鉴定了黑麦草内生真菌。实验证明通过诱导内生真菌产孢的方法并借助形态学依据来鉴定无性的内生真菌是可行的。更多还原

【Abstract】 Six endophytic fungi which were stable in morphology were isolated from five varieties of ryegrass (Lolium perenne L.). The mycelia sterilia were inoculated onto plates containing PDA and MEA and treated with pre-incubation, mycelium washing, being incubated under UV-light and placing leaves of the host grass onto mycelia. After treatments, some strains formed conidia. The endophytic fungi in L. perenne then could be successfully identified according to the characters of the conidia. Some endophytic mycelia sterilia can be identified by using this method.更多还原