【摘要】 以一株产耐高温α-淀粉酶菌株(地衣芽孢杆菌)为出发菌株,经过原生质体制备后由NTG(亚硝基胍)药物诱变,从大量突变株中筛选获得一株高产、稳定的耐高温α-淀粉酶产生菌。摇瓶发酵酶活由选育前的2800u/ml提高到了4100u/m左右,提高了近45%。更多还原

【Abstract】 A thermo stable α-Amylase producer strain(Bacilus Licheniformis)was selected out from a lot of mutant strain by using protoplast preparation and mutagenic treatment with N-methyl-N’-nitrosoguanidine(NTG).4100u/ml of thermo stable αAmylase was accumulated in shaking flask more than 45% compared with the origin.更多还原