【摘要】 目的　评价局部动脉内灌注碱性成纤维细胞生长因子 (bFGF)改善兔部分缺血的血管重建、血液灌流和肢体功能的功效。方法　选用成年雄性新西兰大白兔 2 7只 ,外科结扎右股动脉各主要分支 ,制成右后肢部分缺血模型。每只兔子的左后肢作为非缺血对照组。 2 7只兔子随机分成 3组 ,动脉内灌注bFGF组 (n =9) ,静脉内灌注bFGF组 (n =9)和动脉内灌注生理盐水组 (n =9)。在动物模型制备当即及术后 8、15d分别给药或生理盐水 1次 ,每次经动脉或静脉内给予bFGF的量为 10 μg(4ml) ,或静脉给予同等量的生理盐水。各治疗组间以及组内缺血与非缺血肢体间的差异由以下指标评价 :①术后2 2d缺血区肌肉组织的血管断面数 (VSC)、血管断面面积 (VSS)和血管断面周长 (VSP)的测算 ;②缺血肢体毛细血管再充盈时间的测定 ;③缺血肢体的功能和营养的观察。各组间的统计差异使用单侧检验的方差分析和t检验。结果　动脉内灌注bFGF组的VSC、VSS及VSP值较静脉内给药组和生理盐水组显著增高 (P <0 .0 1)。术后 2 2d ,所有动脉内灌注bFGF组缺血肢体的毛细血管再充盈时间、新毛生长、功能及外观均接近正常 ;而生理盐水组的肢体缺血性表现、毛细血管再充盈时间和肢体功能却无明显改善。静脉内给药组的各项评价指标与生理盐水组无统计学差?更多还原

【Abstract】 Objective To evaluate the effect of intra-arterial infusion of basic fibroblast growth factor(bFGF) on improving neovascularization, vascular perfusion and the function of partially ischemic limbs of rabbits. Methods Twenty-seven New Zealand male rabbits were selected. Partial ischemia model was induced by surgical ligation of the primary branches of right femoral artery in each animal, and the left hind limb of each animal was served as a nonischemic control. Then, 27 rabbits were randomly assigned to three groups: intra-arterial (IA) infusion of bFGF (n=9), intravenous (IV) infusion of bFGF and IA infusion of saline (n=9). Infusion was separately performed immediately after vascular ligation, 8th and 15th days post-surgery with 10 μg (4 ml) of bFGF per-time (or the same volume of saline). The differences between three groups and between ischemic and nonischemic limbs of the same group were compared and evaluated by the following indexes: (1) vessel section count (VSC), vessel section surface area (VSS) and vessel section perimeter (VSP) in the field of ischemic muscle tissues taken at 22nd day postoperatively; (2) capillary refilling time of ischemic limbs; and (3) functional and trophic changes of ischemic limbs. Statistical differences were evaluated by one-way ANOVA and T test. Results VSC, VSS and VSP of the IA-bFGF group were significantly increased than those of the IV-bFGF and IA-saline groups (P< 0.01). At 22nd day postoperatively, the capillary refilling time, new hair growth, the appearance and function of all ischemic limbs in IA-bFGF group were approximately normal. However, in IA-saline group, the ischemic changes, capillary refilling time and the function of ischemic limbs were not improved significantly. All the indexes of IV-bFGF group showed no difference statistically from those of IA-saline group. Conclusions This experimental study identifies that intra-arterial infusion of bFGF may significantly promote neovascularization and vascular perfusion in ischemic limbs, suggesting that a better effect may be obtained with IA-infusion bFGF than that of IV-infusion bFGF.(J Intervent Radiol, 200更多还原