【摘要】 目的:了解广西融水苗族人群9个短串联重复序列(STR)D3S1358、vWA、FGA、D8S1179、D21S11、D18S51、D5S818、DO3S317、D7S820遗传多态性分布情况。方法:用枸橼酸钠抗凝法采集融水苗族个体的血样,酚-氯仿抽提法提取DNA,荧光标记复合扩增基因座,用ABI Prismr(?)3100型遗传分析仪对扩增产物进行检测。结果:9个STR位点共检测出97种等位基因、297 种基因型,基因型的分布符合Hardy-Weinberg平衡定律,平均杂合度0.786,累积个人识别力0.999 999 999 97,累积非父排除率0.999 998,多态信息总量0.999 998 89。结论:苗族群体的9个STR位点具有高度多态性和遗传稳定性,可作为广西苗族的民族遗传学研究和法医学鉴定的基础数据。更多还原

【Abstract】 Objective: To investigate the distributions of nine short-tandem repeat loci in Miao nationality at Rongshui country in Guangxi province. Methods: Sodium-citrated blood specimens were collected from Miao individuals in Rongshui country. The DNAs from the specimens were extracted with phynol-chloroform method, PCR Amplification Kit was used to amplify the extracted DNAs, and 3100 Genetic Analyzer was used to analyse and screen the amplified products. Results: Totally 97 alleles and 297 genotypes of the nine short-tandem repeat loci were found in 208 specimens. The expected genotype distributions accorded with Hardy-Weinberg equilibrium. Total discrimination power, cumulative exclusion power, total polymorphism information and average expected heterozygosity were respectively 0. 999 999 999 97 and 0. 999 998, 0. 999 998 89, 0. 786. Conclusion: The nine short-tandem repeat loci of Miao nationality group show high polymorphism and hereditary stability, accord with Mendel’s law. The a-bove data obtained are valuable in population genetics research, forensic application, and individual identifications.更多还原