【摘要】 目的:探讨雌激素受体(ER)α、β亚型的表达量在子宫平滑肌瘤发病中的意义。方法:取自愿手术的子宫肌瘤患者30例为研究对象,应用非放射性原位杂交技术方法测定平滑肌瘤组织中ERα和ERβmRNA的表达,并与正常的平滑肌组织相比较。结果:ERαmRNA和ERβmRNA主要在平滑肌细胞中表达。子宫肌瘤和正常的平滑肌组织中ERαmRNA的表达量高于ERβmRNA;ERαmRNA在子宫肌瘤中的表达明显高于正常的平滑肌组织,而ERβmRNA的表达仅呈下降趋势。结论:子宫肌瘤的发生发展可能与ERαmRNA的高表达有关,亦可能与ERβmRNA减少趋势有关。更多还原

【Abstract】 Objective: To study the expression of estrogen receptor (ER)-α and -β messenger RNA(mRNA) in uterine leiomyomas. Methods: Samples of uterine leiomyomas and myometriums were obtained from the patients during surgery. Expression of ER-α and ER-β mRNA was detected with nonradioactive in situ hybridization (ISH) techniques. Results: ER-α and ER-β mRNA were mainly localized in uterine smooth muscle cells, but ER-α was significantly higher than ER-β. Expression of ER-α mRNA in leiomyomas was higher than that in myometrium. The expression of ER-β showed a decreasing trend in leiomyoma. Conclusion: The increase of ER_ α mRNA expression and decrease of ER_ β mRNA expression are related to the development of leiomyoma. The imbalance in expression of ER subtypes contributes to the pathogenesis of leiomyoma.更多还原