【摘要】 目的观察正常人皮肤成纤维细胞体外培养过程中超微结构的改变。方法将下2人二倍体成纤维细胞进行连续传代培养,以不同代次的细胞为实验对象,通过倒置相差普微镜与透射电镜对各代细胞进行观察。结果45代以内的细胞生长较迅速,45代后的细胞生长逐渐缓慢,65代细胞几乎无增殖趋势。40代以内细胞的超微结构基本正常,41~65代细胞出现核膜内折,细胞核/浆比例减小,细胞表面突起和微绒毛减少。结论40代以内成纤维细胞适合做组织工程的种子细胞。更多还原

【Abstract】 Objective To investigate the alteration of the ultrastructure of fibroblasts isolated from human skin. Methods Fibroblasts from human normal skin were taken for successive cell culture. The aging processs was displayed by phase reverse microscope and ultrastructure observed by transmission electron microscope. Results Cells below 45 passages proliferated rapidly, those from 45 to 60 passages grew slowly gradually and those beyond 61 passages showed significant senescence. No significant change in ultrastructure was observed in cells below 40 passages, but great alteration was found beyond 41 passages. Conclusion Fibroblasts before 40 passages are suitable for seeds cells for skin tissue engineering.更多还原