【摘要】 为了解近江牡蛎肠道厌氧菌在食饵消化过程中的作用,为微生态饲用添加剂的开发研究打下基础,从近江牡蛎肠道中分离出8株厌氧菌,其中有5株为革兰氏阳性菌。并研究了它们蛋白酶、脂肪酶、淀粉酶、纤维素酶的产酶能力,结果表明仅有3株菌具有产酶能力:2株菌能分泌脂肪酶,1株菌能分泌蛋白酶。由此可见,厌氧菌在分泌消化酶、消化食饵中所起的作用不大。更多还原

【Abstract】 In order to understand the role(s) of anaerobic bacteria from the oyster intestine in the digestion of food and to optimize the microbial composition so as to make more efficient use of fish feed,anaerobic bacteria from Ostrea vivularis Gould intestine were isolated and their enzyme-producing capabilities were investigated.In total,8 strains of anaerobics were isolated and Gram stain showed that 5 strains are Gram-positive while 3 Gram negative. Casein,olive oil,starch and CMC were used as substrates for screening protease,lipase,amylase and cellulase respectively.Results showed that only 3 strains possessed digestive enzyme producing abilities:2 strains produced lipase while 1 strain produced protease. Therefore,it can be concluded that anaerobic bacteria in the intestine of Ostrea vivularis Gould contribute little to the digestion of food.更多还原