【摘要】 目的:观察重组人卵透明带蛋白(rhZP3)对人精子顶体反应的诱导作用。方法:正常生育力人精子经过获能后,分别用BSA、孕酮和含不同浓度的重组人ZP3蛋白的培养液共孵育,诱导精子顶体反应,对实验组和对照组的精子的顶体发生状态用考马斯亮蓝染色法进行评价。结果:精子与培养液共孵育30 min后,rhZP3组与BSA组之间差异有显著性(P<0.05),顶体反应率随rhZP3质量浓度增加而升高。结论:重组人ZP3蛋白对人精子顶体反应有显著的诱导效应,且在一定范围内与质量浓度成正相关,重组人ZP3蛋白具有发展精子顶体反应试剂盒的潜能。更多还原

【Abstract】 Aim: To detect the capacity of recombinant human zona pellucida-3 protein(rhZP3) in inducing acrosome reaction of human sperm.Methods: Semen samples were obtained by masturbation after 2 to 3 days’ abstinence from men with normal parameters on semen analysis according to World Health Organization criteria.After capacitation,the motile sperm were divided into groups which was separately incubated with BSA medium,progestone and different concentrations of rhZP3 to induce acrosome reaction(AR).The acrosome status of sperm was evaluated by Coomassie brilliant blue(CBB) staining. Result: After 30 minutes incubation in the medium,the sperm showed a significant difference between the rhZP3 group and the BSA group(P<0.05) and the AR rate increased with the concentration of rhZP3.Conclusion: rhZP3 can induce the acrosome reaction and the percentage AR of sperm is correlated with the concentration of rhZP3 to some extent.These results suggest that the rhZP3 has the potential of development of diagnostic kits for the AR.更多还原