【摘要】 目的:比较肝癌BEL-7402和SMMC-7721细胞对三氧化二砷(As2O3)诱导凋亡的敏感性差异,探讨细胞内谷胱甘肽(GSH)含量与其作用的关系。方法:不同浓度As2O3作用BEL-7402和SMMC-7721细胞24~96 h,流式细胞仪检测细胞DNA含量,计算细胞凋亡百分率;GSH检测试剂盒检测细胞内GSH含量。结果:0.25μmol/L As2O3作用24 h,有效地诱导BEL-7402细胞凋亡;对SMMC-7721细胞来说,需要用1.0μmol/L As2O3作用24 h才能达到相同的抑制效果。1.0μmol/LAs2O3作用72 h时,BEL-7402细胞的凋亡率为(43.8±0.2)%,SMMC-7721细胞的凋亡率为(12.8±0.2)%,检测BEL-7402细胞内GSH为(18.7±1.4)nmol/mg;SMMC-7721细胞内GSH为(50.8±5.2)nmol/mg,两者比较均有显著差异。结论:肝癌BEL-7402和SMMC-7721细胞对As2O3诱导细胞凋亡的敏感性不同,可能与细胞内GSH含量有关。更多还原

【Abstract】 Aim: To compare the susceptive differences of arsenic trioxide （As₂O₃） induced apoptosis on BEL-7402 and SMMC-7721 cells, and explore the effect of GSH level in cells on them. Methods: The flowcytometry was used to detect the apoptotic percentage and GSH kit to determine the level of GSH while the effect of arsenic trioxide on cells of BEL-7402 cells and SMMC-7721 cells reached 24-96 hours. Results: Arsenic trioxide is able to induce apoptosis of BEL-7402 cells at 0.25 μmol/L for 24 hours;however, it needed 1.0 μmol/L of As₂O₃ for 24 hours to produce the similar results for SMMC-7721 cells. The apoptotic rate of BEL-7402 cells is （43.8±0.2）% at dose of 1.0 μmol/L As₂O₃ for 72 hours, and the apoptotic rate of SMMC-7721 is （12.8±0.2）% only; at this time, level of GSH in BEL7402 cells is （18.7±1.4） nmol/mg, and in SMMC-7721 cells is （50.8±5.2） nmol/mg, which showed the significant difference between them. Conclusion: The sensibility difference between BEL-7402 and SMMC-7721 hepatocarcinoma cells to As₂O₃ are related with level of GSH in cells.更多还原