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显齿蛇葡萄组培育苗试验
Tissue Culture Propagation in Ampelopsis grossedentata
【摘要】 为增加显齿蛇葡萄的繁殖速度,通过组培试验培养显齿蛇葡萄无病毒苗,结果表明:显齿蛇葡萄外植体脱分化以MS+6-BA1.5mg.L-1的浓度的愈伤组织生长最快;在显齿蛇葡萄分化芽的过程中,不同处理组合中以MS+6-BA1.5mg·L-1+NAA0.05mg·L-1表现最好;在显齿蛇葡萄生根培养中,不同处理以1/2N6+6-BA1.5mg·L-1+NAA0.15mg·L-1表现最好。
【Abstract】 With a view to accelerating propagation of Ampelopsis grossedentata. tissue culture was conducted to produce virus -free planting material. Experiments indicated that the callus induced from the leaf explants cultured on the MS medium with 1. 5 mg·L-1 of 6-BA showed the fastest growth. In bud differentiation, the MS medium with 1. 5 mg·L-1 of 6-BA and 0. 05 mg·L-1 of NAA generated the best result. The N6 mediums with 1. 5 mg·L-1 of 6-BA and 0. 15 mg·L-1 of NAA achieved the highest rooting rate.
【关键词】 显齿蛇葡萄;
组织培养;
生长促进物质;
【Key words】 Ampelopsis grossedentata; tissue culture; growth acceleration hormone;
【Key words】 Ampelopsis grossedentata; tissue culture; growth acceleration hormone;
- 【文献出处】 经济林研究 ,Economic Forest Researches , 编辑部邮箱 ,2005年02期
- 【分类号】S663.1
- 【被引频次】9
- 【下载频次】206