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柔嫩艾美耳球虫杂交株MIC2基因克隆及序列分析

Cloning and Sequencing of MIC2 Gene from Hybridization Strain of Eimeria tenella

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【Author】 WANG Jing-min~1, WEI Feng~(2,3), ZHANG Xi-chen~2, CAI Ya-nan~3, LIU Quan~(2,4),WU Tao~2, LI Jian-hua~2, et al. (1.Information Mangement of Changchun Taxation College,Changchun 130021,China; 2. College of Veterinary Medicine, Jilin University, Changchun 130062, China; 3. College of Biotechnology, Jilin Agricultural University, Changchun 130118, China; 4. Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130062, China)

【机构】长春税务学院信息管理系；吉林大学畜牧兽医学院；吉林农业大学生物技术学院；吉林大学畜牧兽医学院长春130021；长春130062；长春130118；长春130062；军事医学科学院军事兽医研究所；

【摘要】根据柔嫩艾美耳球虫子孢子微线蛋白2的cDNA序列,利用计算机设计1对引物,以柔嫩艾美耳球虫杂交株子孢子总RNA为模板,利用RT-PCR方法扩增出1个片段。把这个片段克隆到pMD18-T载体,经酶切鉴定得到1个阳性克隆,测序及序列分析结果表明该片段为MIC2基因,开发阅读框(ORF)与E.tenella豪顿株MIC2核苷酸同源性为99.51%,推导的氨基酸序列同源性为99.12%,说明MIC2基因高度保守。更多还原

【Abstract】 The MIC2 gene of E.tenella hybridization strain was amplified with the primers designed according to MIC2 gene cDNA of E.tenella and cloned into pMD18T vector. The recombinant plasmid was identified by restriction endonuclease EcoRⅠ/HindⅢ and sequenced.The results indicated that the MIC2 gene of E.tenella hybridization strain including 1029 bp nucleotides was successfully cloned and there were only five nucleotides different from that of E.tenella Horton strain and it was very conservative in that the homogeneity of nucleotide and amino acid was respectively 99.51% and 99.12%.更多还原

【关键词】柔嫩艾美耳球虫；微线蛋白；基因克隆；序列分析；
【Key words】 Eimeria tenella； MIC； gene cloning； sequencing；

【基金】国家自然科学基金资助项目(30170696)

【文献出处】吉林农业大学学报 ,Journal of Jilin Agricultural University , 编辑部邮箱 ,2005年04期

【分类号】S852.723
【被引频次】5
【下载频次】188

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