【摘要】 以"米邦塔"食用仙人掌幼掌为外植体,研究了不同取材方法及不同培养基对其芽诱导及再生的影响。结果表明:适于"米邦塔"食用仙人掌芽诱导分化的培养基为MB+6 BA1.0mg L+NAA0.2mg L,继代增殖的培养基为MB+6 BA0.5mg L+NAA0.1mg L,生根培养基为1 2MS+NAA0.3mg L;用3～5日龄掌片的上部或顶部作外植体诱导效果较好,小掌的中下部继代增殖率较高;移栽基质以河沙为最好,成活率达90.1%。更多还原

【Abstract】 <Abstrcat> Using the tender shoots as the explants, the effects of different ways of selecting materials and media on bud induction and plant regeneration are examined. The results showed that the appropriate medium for bud induction is MB+6-BA 1.0 mg/L+NAA 0.2 mg/L, the medium of multiplication is (MB+)6-BA 0.5 mg/L+NAA 0.1 mg/L, and the medium of rooting is 1/2 MS+NAA 0.3 mg/L. The effect of propagation is better when 3～5　day upper or top buds are used as explants.The multiplication of the middle and lower parts of the micro-cladode is higher. The best transplantation medium is sand and it is up to 90.1% survival.更多还原