【摘要】 目的探讨重组CTLA4Ig及IκBα重组腺病毒载体延长烧伤创面皮片移植存活情况。方法采用Wistar大鼠36只,随机分为3组,1组:正常对照组,2组:用AdCTLA4Ig液室温浸泡转染1h,3组:用Adv CD40LIg-IRES2-CTLA4Ig液室温浸泡转染1h。麻醉后常规背部备皮,在4cm×4cm面积上涂3%凝固汽油2ml,燃烧20s,致大鼠背部皮肤Ⅲ度烧伤,于烧伤后第3天,将所制备1.0cm×2.0cm大小长方形皮瓣移植于创面,加压包扎。观察重组腺病毒载体转染移植物后,在大鼠背部烧伤创面移植人断层皮肤的存活情况的影响。结果重组腺病毒载体能延长大鼠烧伤创面移植人断层皮肤的存活时间。结论重组腺病毒可用于延长烧伤创面异基因移植皮肤的存活期。更多还原

【Abstract】 Objective To observe the effect of recombinan t expression vector of CTLA4Ig and IκBα on the survival time of xenoskin graft o n burn wound. Methods A total of 36 Wistar rats established as Ⅲ degree burn on the back were randomly divided into 3 groups (n=12 in each gro up): normal control (the xenoskin without any special treatment), AdCTLA4Ig grou p (the xenoskin was transfected with AdCTLA4Ig), and Adv CD40LIg-IRES2-CTLA4Ig g roup [the xenoskin was transfected with the recombinant adenovirus expression v e ctor of CTLA4Ig and IκBα (Adv CD40LIg-IRES2-CTLA4Ig) that was constructed by m eans of homorecombination]. After the human skin grafts were transplanted to th e burned rats, the survival time and biopsy of grafted skins were observed. Results The recombinant adenovirus expression vector of CTLA4Ig and IκBα could prolong the survival time of the grafted xenoskins on burn wounds i n rats. Conclusion The recombinant Ad-CTLA4Ig-IRES(2) Iκ B α could be used to prolong the survival time of the grafted xenoskins on burn w ounds.更多还原