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光合细菌M-01的分离及培养条件研究

The Isolation and Preliminary Culture Condition Study of Photosynthetic Bacterium M-01

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【作者】 操玉涛陈刚刘立鹤肖明禅叶剑陈佳

【Author】 CAO Yu-tao,CHEN Gang (Fisheries College, Zhanjiang Ocean University, Zhanjiang, Guangdong 524025, China)LIU Li-he (Zhanjiang Aquafeed Technology Center,Zhanjiang, Guangdong 524017, china)XIAO Ming-chan,YE Jian,CHEN Jia (Fisheries College, Zhanjiang Ocean University, Zhanjiang, Guangdong 524025, China)

【机构】 湛江海洋大学水产学院湛江水产饲料技术中心湛江海洋大学水产学院 广东湛江524025广东湛江524025广东湛江524017广东湛江524025

【摘要】 从湛江市霞山区海滩表层污泥中富集分离出红假单胞菌属光合细菌M-01,对其培养条件进行了初步探讨,结果表明:菌株生长温度为30℃时,最适培养光照度为3000lx。培养基正交试验结果表明其最优培养基配方为:氯化铵2g,磷酸氢二钾0.2g,乙酸钠4g,碳酸氢钠2g,氯化钠1g,酵母膏0.15g,硫酸镁0.2g,T.M储液少量,蒸馏水1000mL;其中乙酸钠为最大影响因子。

【Abstract】 The photosynthetic bacterium M-01 was isolated from the sludge surface of the beach in Xiashan district of Zhanjiang City and identified as belonging to Rhodopseudomonas. The optimal culture light was 3 000 lx when the growth temperature was 30 ℃. An orthogonal experiment showed that the optimal medium ingredients were NH4Cl 2 g, KH2PO40.2 g, sodium acetic 4 g, NaHCO3 2 g, yeast paste 0.15 g, NaCl 1 g, MgSO4 0.2 g, some trace elements (B, Mn, Fe, Mo, Zn), stilled water 1 000 mL. And sodium acetic was the most influencing factor among the ingredients.

  • 【文献出处】 长江大学学报(自科版) ,Journal of Yangtze University(Natural Science Edition) , 编辑部邮箱 ,2005年08期
  • 【分类号】Q93-33
  • 【下载频次】231
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