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光合细菌M-01的分离及培养条件研究
The Isolation and Preliminary Culture Condition Study of Photosynthetic Bacterium M-01
【摘要】 从湛江市霞山区海滩表层污泥中富集分离出红假单胞菌属光合细菌M-01,对其培养条件进行了初步探讨,结果表明:菌株生长温度为30℃时,最适培养光照度为3000lx。培养基正交试验结果表明其最优培养基配方为:氯化铵2g,磷酸氢二钾0.2g,乙酸钠4g,碳酸氢钠2g,氯化钠1g,酵母膏0.15g,硫酸镁0.2g,T.M储液少量,蒸馏水1000mL;其中乙酸钠为最大影响因子。
【Abstract】 The photosynthetic bacterium M-01 was isolated from the sludge surface of the beach in Xiashan district of Zhanjiang City and identified as belonging to Rhodopseudomonas. The optimal culture light was 3 000 lx when the growth temperature was 30 ℃. An orthogonal experiment showed that the optimal medium ingredients were NH4Cl 2 g, KH2PO40.2 g, sodium acetic 4 g, NaHCO3 2 g, yeast paste 0.15 g, NaCl 1 g, MgSO4 0.2 g, some trace elements (B, Mn, Fe, Mo, Zn), stilled water 1 000 mL. And sodium acetic was the most influencing factor among the ingredients.
【Key words】 photosynthetic bacteria; isolation; culture; orthogonal experiment;
- 【文献出处】 长江大学学报(自科版) ,Journal of Yangtze University(Natural Science Edition) , 编辑部邮箱 ,2005年08期
- 【分类号】Q93-33
- 【下载频次】231