【摘要】 目的:探讨人胚食管上皮永生化细胞株SHEE、SHEEMT、食管癌细胞株EC8712中PTEN表达的差,判断食管上皮细胞在恶性转化过程中是否有PTEN缺失现象的发生;方法:培养三种细胞株,采用免疫组织化学、激光共聚焦显微镜、流式细胞仪和Westernblot等检测方法对三种细胞株中PTEN的表达进行检测。结果:三种细胞株均有PTEN的表达,表达强度与分化程度有关,PTEN在三种细胞株中表达强弱顺序为SHEE>SHEEMT>EC8712,差异有统计学意义(P<0.01);结论:PTEN在SHEE、SHEEMT和EC8712三种来源于食管鳞状上皮但分化程度不同的细胞株均中表达,表达强度与分化程度相关,分化程度越高,表达越强。更多还原

【Abstract】 Objective:The research aims to detect expression of tumor suppressor PTEN in three types of cell line to study the relationship between its expression with differentiation of three cell lines originating from esophageal squamous epithelial cell. Methods: Three types of cell line were cultivated and detected with the SABC immunohistochemistry,laser confocal microscope,flow cytometry and western blotting methods for the oncogene PTEN expression.Results:1.Positive stainings were found in the cytoplasma as well as in the nucleus in three cell lines by immunohistochemistry and laser confocal microscope; 2.The expression of PTEN was related to differentiation grade of cell lines,the well-differentiation SHEE cell line expressed the strongest, while the esophagus cancer cell line EC8712 expressed the lowest,the difference is significant (P<0.01);Conclusions:The PTEN protein was expressed in three types of cell line.The level of PTEN expression was related to differentiation degree.更多还原