【摘要】 目的建立高效液相色谱法测定氯霉素甲硝唑洗鼻剂中氯霉素、甲硝唑含量测定方法及其有关物质氯霉素二醇物、对硝基苯甲醛的定量检查方法。方法采用高效液相色谱法。Intersil C18分析色谱柱(4.6mm ID×250mm,粒径5μm),流动相:甲醇-水-三乙胺(480∶520∶7,含1%磷酸二氢钠,0.2%庚烷磺酸钠,磷酸调pH值为3.0);流速1.0ml/min;检测波长270nm。结果甲硝唑、氯霉素、氯霉素二醇物和对硝基苯甲醛的理论板数分别为4 700、4 5004、400、1 500。回归方程分别为:Y=19156 100.5X+424 145.7(r=0.999 9),Y=65 390 024.2X-5 660.6(r=0.999 9),Y=39 817 070.9X+1 373 589.3(r=0.999 8),Y=83 051 977.5X-284 191.7(r=0.999 8);线性范围分别为0.423 0~2.115μg,0.450 0~2.250μg,0.660 0~3.300μg,0.444 0~2.220μg;平均回收率和RSD分别为100.8%,1.4%;99.2%,1.2;101.1%,1.5;99.3%,1.6;最低检测限分别为0.002,0.002,0.002和0.003μg。结论用高效液相色谱法测定氯霉素甲硝唑洗鼻剂中甲硝唑、氯霉素二醇物、氯霉素、对硝基苯甲醛的含量,操作简便,结果准确。更多还原

【Abstract】 Aim A quantitative method was established for the determination of Chloramphenicol,Metronidazole,2-Amino-1-（4-nitrophenyl）propane-1,3-diol and 4-Nitrobenzaldehyde in Collunarium by means of HPLC.Method The chromatographic conditions includes Intersil C₁₈ column and mobile phase consist of methanol-water-triethylamine（480∶520∶7,containing 1% NaH₂PO₄,0.2% 1-heptanesulfonate,sodium salt,to regulate pH to 3.0 with phosphoric acid）.The flow rate is 1.0ml/min.The detective wavelength was 270 nm performed at room temperature.Results The number of theoretical plates caculated by metronidazole,chloramphenicol 2-Amino-1-（4-nitrophenyl）propane-1,3-diol and 4-Nitrobenzaldehyde peak were 4 700,4 500,4 400 and 1 500 respectively;the regression equations were Y=19 156 100.5X+424 145.7（r=0.999 9）,Y=65 390 024.2X-5 660.6（r=0.999 9）,Y=39 817 070.9X+1 373 589.3（r=0.999 8）,Y=83 051 977.5X-284 191.7（r=0.999 8） respectively,the linear ranges were 0.423 0～2.115μg,0.4500～2.250μg,0.6600～3.300μg,0.444 0～2.220μg respectively;the average recovery RSD were 100.8%,1.4%;99.2%,1.2%;101.1%,1.5%;99.3%,1.6% respectively;the measurable lowest limit were 0.002,0.002,0.002,0.003μg respectively.Conclusion The method is rapid,accurate and reliable.更多还原