【摘要】 目的探讨己酮可可碱(pentoxifylline,PTX)对新生大鼠缺氧缺血性脑病(hypoxic-ischemicencephalopathy,HIE)脑组织中ATP酶和炎性细胞因子的影响,揭示PTX对大鼠HIE的保护机制。方法7日龄Wistar大鼠80只随机分为假手术组、缺血组、低氧组、HIE模型组和PTX组。缺血72h后测定脑组织中Na+、K+-ATP酶、Ca2+-ATP酶和Mg2+-ATP酶活性,以及TNF-αI、L-1和TXB2含量。结果缺血组、低氧组和HIE模型组Na+、K+-ATP酶、Ca2+-ATP酶和Mg2+-ATP酶活性降低(P<0.01),TNF-αI、L-1和TXB2含量升高(P<0.05,P<0.01)。PTX组可显著缓解上述变化(P<0.05,P<0.01)。结论PTX对新生大鼠HIE的保护作用可能与其保护脑组织中ATP酶和降低炎性细胞因子有关。更多还原

【Abstract】 ObjectiveTo explore the effects of pentoxifylline（PTX） on ATPase and inflammatory cytokines in cerebral tissue and to identify the mechanism of PTX protection in newborn rats with hypoxic-ischemic encephalopathy. （MethodsWistar） rats（80 case） were randomly divided into sham-operation （Sham） group, ischemia （I） group, hypoxia （H） group, hypoxic-ischemic encephalopathy model （HIE） group and PTX group. After hypoxic-ischemic processing, the activities of Na⁺, K⁺-ATPase, Ca²⁺-ATPase and Mg²⁺-ATPase and the content of TNF-α,IL-1 and TXB₂ in cerebral tissue were measured. ResultsThe activities of Na⁺, K⁺-ATPase, Ca²⁺-ATPase and Mg²⁺-ATPase in the I group,H group and HIE group were significantly decreased and the contents of TNF-α?IL-1 and TXB₂ were markedly increased （P<0.05, P<0.01） as compared to the Sham group. PTX attenuated those changes markedly （P<0.05, P<0.01）.ConclusionThe results demonstrate the protective effect of PTX against hypoxic-ischemic encephalopathy in rats which is possibly related to the protection of activities of ATPase to the decreasing of inflammatory cytokines in cerebral tissue.更多还原