【摘要】 目的筛选与白细胞介素2受体(IL2R)α基因NIRS元件相互作用的结合蛋白。方法采用酵母单杂交体系从HTLV1转化的人外周血淋巴细胞MATCHMAKERcDNA文库中筛选与NIRS相互作用的蛋白。结果经过筛选并排除假阳性后有9个阳性克隆仍保持His+表型和βgal活性;测序后分析发现它们分别属于4个不同蛋白的cDNA克隆。其中一个是已知的反式作用因子Ku抗原,另一个是RNA聚合酶Ⅰ的转录终止因子。它们的C末端分别含有SAP和SANTDNA结合结构域。结论在Jurkat细胞中存在与NIRS元件相互作用的结合蛋白,相关的研究正在进行中。更多还原

【Abstract】 Objective To screen the binding proteins of Human Interleukin-2 receptor α Chain NIRS element. Methods Yeast one-hybrid system was used to screen NIRS binding proteins in HTLV-1 transformed Jurkat cell cDNA fusion library. Results There are nine clones have the His + epitope and β-gal activity after the secondary screening. The sequencing results indicate that they are belonging to 4 different proteins. Among them, a known trans-acting factor Ku antigen and transcription termination factor of RNA polymerase I (TTF1) contain SAP domain and SANT domain, respectively. They are DNA binding domains. Conclusion Four candidate clones encoding for NIRS binding proteins were obtained from Jurkat cells . The regulation mechanism of these candidate proteins in IL-2R α gene is undergoing.更多还原